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The characterization of groundwater movement is a key element to all groundwater and surface water investigations. Yet, tracing the movement of groundwater with fluorescent tracer dyes is a simple but under-utilized investigative method in hydrogeology. This book offers recommendations for ways to conduct various facets of groundwater tracing using fluorescent tracer dyes. Recommendations are intended as general guidance rather than being firm rules. In an era where standardization and procedural manuals are in vogue, it must be remembered that there are multiple ways to approach a problem. The range of conditions and issues that confront those who are considering a groundwater tracing project are extremely diverse. As a result, no detailed standardized approach or procedure will be the best method in all cases. Accordingly, for essentially every recommendation we present in this book there can be exceptions. To demonstrate the wide range of practical conditions of tracer studies, 35 case studies are presented to provide specifics of how the tracing work was conducted and the associated results. Ultimately, this book aims to help people involved with groundwater issues appreciate situations where groundwater tracing with fluorescent tracer dyes is appropriate, and to understand practical approaches, methods, and key study design aspects for conducting tracing work.